Incamake | Kumenya ibintu byihariyeIkirenge n'umunwa antibodyAndika O. |
Ihame | Ubwoko bwa FMD Ubwoko bwa Aziya I antibody ELISA yifashisha mugushakisha antibodiyite zanduza ibirenge n umunwa muri serumu yingurube, inka, intama n'ihene kugirango hasuzumwe ubudahangarwa bw'inkingo ya FMD. |
Intego zo Kumenya | Ikirenge n'umunwa antibodyAndika O. |
Icyitegererezo | Serumu
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Umubare | 1 kit = 192 Ikizamini |
Guhagarara no Kubika | 1) Reagent zose zigomba kubikwa kuri 2 ~ 8 ℃.Ntukonje. 2) Ubuzima bwa Shelf ni amezi 12.Koresha reagent zose mbere yitariki yo kurangiriraho kubikoresho.
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Virusi yindwara yibirenge(FMDV) niindwaraibyo biteraindwara y'ibirenge n'umunwa.[1]Ni apicornavirus, prototypical umunyamuryango wubwokoAphthovirus.Indwara, itera imitsi (ibisebe) mu kanwa no mu birenge byainka, ingurube, intama, ihene, nizindiibinonoinyamaswa zirandura cyane kandi nicyorezo gikomeyeubworozi bw'amatungo
IyiKituseindirectELISAmethod, isukuyeFMDVantigenispre-yometse kuri enzyme ya micro-neza.Mugihe cyo kwipimisha, wongeyeho serumu ntangarugero, nyuma yubushakashatsi, niba hari antibody yihariye ya virusi ya FMD, izahuza na antigen yabanje gutwikirwa, ijugunye antibody idahujwe nibindi bikoresho hamwe no gukaraba;hanyuma ongeramo enzyme conjugate, ujugunye enzyme idahuye hamwe no gukaraba.Ongeramo TMB substrate muri micro-iriba, ikimenyetso cyubururu na Enzyme catalysis ni igipimo cyibintu bya antibody murugero.
Reagent | Umubumbe 96 Ibizamini / 192Ibizamini | ||
1 |
| 1ea / 2ea | |
2 |
| 2ml | |
3 |
| 1.6ml | |
4 |
| 100ml | |
5 |
| 100ml | |
6 |
| 11 / 22ml | |
7 |
| 11 / 22ml | |
8 |
| 15ml | |
9 |
| 2ea / 4ea | |
10 | microplate ya serumu | 1ea / 2ea | |
11 | Amabwiriza | 1 pc |