Ibicuruzwa
Avian Leukemia P27 Antigen ELISA Kit
Indwara ya Hydatid Yanduye Antibody ELISA Kit
| Incamake | ikoreshwa mu kumenya Avian leukose P27 antigen mumaraso yinyoni, umwanda, cloaca, numweru yera. |
| Ihame | Avian Leukose (AL) P27 antigen Elisa kit ikoreshwa mugutahura antigen leukose P27 antigen mumaraso yinyoni, umwanda, cloaca, numweru w'igi.
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| Intego zo Kumenya | Avian Leukose (AL) P27 antigen |
| Icyitegererezo | Serumu
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| Umubare | 1 kit = 192 Ikizamini |
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Guhagarara no Kubika | 1) Reagent zose zigomba kubikwa kuri 2 ~ 8 ℃.Ntukonje. 2) Ubuzima bwa Shelf ni amezi 12.Koresha reagent zose mbere yitariki yo kurangiriraho kubikoresho.
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Amakuru
Avian Leukose (AL) ni ijambo rusange ku ndwara zitandukanye zifata ibibyimba mu nkoko zatewe na virusi ya Avian Leukose (ALV) mu muryango Retroviridae.Iyi ndwara ikwirakwizwa ku isi kandi ifite umuvuduko mwinshi.Irashobora gutera urupfu no gucika intege mu nkoko, kugabanya ubushobozi bw’umusaruro, kandi ni imwe mu ndwara nyamukuru zibangamira cyane iterambere ry’inganda z’inkoko.Iyi ndwara ifite amateka maremare kandi ihora ihura n’abantu bashya, nka virusi ya Avian leukemia subgroup J (ALV-J), yavumbuwe ikamenyekana mu mpera za 1980 mu Bwongereza nkubwoko bushya bwa virusi ya leukemia, itera abantu benshi kwangiza inganda za broiler
Ihame ry'ikizamini
Igikoresho gikoresha sandwich ya ELISA, isukuye anti-avian leukocyte P27 antibody ya monoclonal antibody yabanje gutwikirwa kumirongo ya enzyme ya micro-iriba.Mu kizamini, antigen iri murugero ihambirwa na antibody ku isahani isize, nyuma yo gukaraba kugirango ikuremo antigen idahuza nibindi bice, antibody ya monoclonal antibody yongeweho kugirango ihuze byumwihariko na antigen-antibody complex kuri plaque.hanyuma gukaraba, enzyme idahwitse conjugate ikurwaho, igisubizo cya substrate ya TMB cyongewe kuri microplate, ikimenyetso cyubururu na catalizike ya Enzyme nikigereranyo cyibintu bya antibody murugero.Ongeraho guhagarika igisubizo, Nyuma yo kubyitwaramo, kwinjiza Agaciro muri reaction neza gipimwa nuburebure bwa 450 nm.
Ibirimo
| Reagent | Umubumbe 96 Ibizamini / 192Ibizamini | ||
| 1 |
| 1ea / 2ea | |
| 2 |
| 2.0ml | |
| 3 |
| 1.6ml | |
| 4 |
| 100ml | |
| 5 |
| 100ml | |
| 6 |
| 11 / 22ml | |
| 7 |
| 11 / 22ml | |
| 8 |
| 15ml | |
| 9 |
| 2ea / 4ea | |
| 10 | microplate ya serumu | 1ea / 2ea | |
| 11 | Amabwiriza | 1 pc |
