Avian Leukemia P27 Antigen ELISA Kit

Igikoresho gikoresha sandwich ya ELISA, isukuye anti-avian leukocyte P27 antibody ya monoclonal antibody yabanje gutwikirwa kumirongo ya enzyme ya micro-iriba.Mu kizamini, antigen iri murugero ihambirwa na antibody ku isahani isize, nyuma yo gukaraba kugirango ikuremo antigen idahuza nibindi bice, antibody ya monoclonal antibody yongeweho kugirango ihuze byumwihariko na antigen-antibody complex kuri plaque.hanyuma gukaraba, enzyme idahwitse conjugate ikurwaho, igisubizo cya substrate ya TMB cyongewe kuri microplate, ikimenyetso cyubururu na catalizike ya Enzyme nikigereranyo cyibintu bya antibody murugero.Ongeraho guhagarika igisubizo, Nyuma yo kubyitwaramo, kwinjirira Agaciro muri reaction neza gipimwa nuburebure bwa 450 nm.