Inkoko Yanduye Bursal Indwara Virus Ab Elisa kit

Igikoresho gikoresha uburyo bwa ELISA burushanwe, virusi yanduye yanduye ya bursal yanduye VP2 kuri microplate, kandi irushanwa na antibody anti-VP2 proteyine muri serumu kugirango ikore icyiciro gikomeye ikoresheje antibody anti-VP2 proteine ​​monoclonal antibody.Mu kizamini, antibody ya monoclonal igomba gupimwa hanyuma hakongerwamo poroteyine irwanya VP2, hanyuma nyuma yububikwe, niba icyitegererezo kirimo virusi yanduye yinkoko virusi ya VP2 proteine ​​yihariye, ihuza na antigen ku isahani isize.Gutyo, guhagarika guhuza anti-VP2 protein monoclonal antibody kuri antigen, nyuma yo gukaraba kugirango ikureho antibody idahuza nibindi bice;hanyuma wongereho anti-imbeba enzyme yanditseho antibody ya kabiri kugirango ihuze cyane na antigen-antibody complex kuri plaque yo kumenya;Enzyme idahwitse conjugate ikurwaho no gukaraba;insimburangingo ya TMB yongewe kuri microwell kugirango itezimbere ibara, kandi agaciro ko kwinjiza icyitegererezo kajyanye nabi nibiri muri antibody anti-VP2 ya proteine ​​irimo, bityo bikagera ku ntego yo kumenya antibody anti-VP2 proteine ​​muri sample