Ibicuruzwa
Amagi Tera Syndrome1976 virusi Antibody ELISA Ki
Amagi Tera Syndrome1976 virusi Antibody ELISA Ki
| Incamake | Use kugirango umenye antibody yihariye irwanya EDS76 muri serumu yujuje ubuziranenge. |
| Ihame | Indwara ya Eggs Drop Syndrome 1976 (EDS76) Ab Elisa kit ikoreshwa mugutahura antibody yihariye irwanya EDS76 muri serumu yujuje ubuziranenge.Kugenzura antibody nyuma ya EDS76 immunite na serologi yo gusuzuma indwara muri Avian. |
| Intego zo Kumenya | Antamuntu urwanya EDS76 muri serumu |
| Icyitegererezo | Serumu
|
| Umubare | 1 kit = 192 Ikizamini |
|
Guhagarara no Kubika | 1) Reagent zose zigomba kubikwa kuri 2 ~ 8 ℃.Ntukonje. 2) Ubuzima bwa Shelf ni amezi 12.Koresha reagent zose mbere yitariki yo kurangiriraho kubikoresho.
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Amakuru
Indwara ya Egg Drop Syndrome (EDS-76) n'indwara ikaze yandura iterwa na adenovirus group ya III yo mu bwoko bwa virusi ya Adenoviridae Avian Virus hamwe na hemagglutination.Mu bworozi bumwe na bumwe bw’inkoko, umusaruro w’amagi menshi y’inkoko wagabanutse mu buryo butunguranye, maze amagi ahinduka nk'amagi yoroshye yoroshye, amagi adafite igikonjo, n'amagi yoroheje yakozwe icyarimwe.Inzira zose zindwara zimara ibyumweru 5-6, nyuma yumusaruro w amagi uzamuka buhoro buhoro, ariko biragoye kugera kurwego mbere yo kugabanuka
Ihame ry'ikizamini
Iki gikoresho gikoresha uburyo butaziguye bwa ELISA, antigen ya EDS76 isukuye yabanje gutondekwa kuri enzyme ya micro-neza neza. Kwemeza, adddilutedserumsample, nyuma yubushakashatsi,ifthereisEDS76virusspecificantibody, izahuza na pre-coatedantigen, yirukana antibody hamwe nibindi bikoresho hamwe no gukaraba;hanyuma ongeramo enzyme yanditseho anti-EDS76 virusi ya monoclonal antibody, hanyuma uhuze antibody ya monoclonal na antigen yabanje gutwikirwa;guta enzyme idahujwe conjugate hamwe no gukaraba;Ongeramo TMB substrate muri micro-mariba, ikimenyetso cyubururu na Enzyme catalysis iri muburyo butandukanye bwibintu bya antibody murugero, koresha umusomyi wa ELISA kuri 450nm wavelenth kugirango upime iyinjizwa Agaciro mumariba ya reaction nyuma yo kongeramo igisubizo cyo guhagarika
guhagarika reaction.
Ibirimo
| Reagent | Umubumbe 96 Ibizamini / 192Ibizamini | ||
| 1 |
| 1ea / 2ea | |
| 2 |
| 2.0ml | |
| 3 |
| 1.6ml | |
| 4 |
| 100ml | |
| 5 |
| 100ml | |
| 6 |
| 11 / 22ml | |
| 7 |
| 11 / 22ml | |
| 8 |
| 15ml | |
| 9 |
| 2ea / 4ea | |
| 10 | microplate ya serumu | 1ea / 2ea | |
| 11 | Amabwiriza | 1 pc |
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